Enzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide.

DPNH-X was prepared as described previously (1) ; the barium salt was converted to the sodium salt before use, and solutions were stored at 20”. Concentrations of DPNH-X were calculated with an extinction coefficient of 13.5 X lo6 sq. cm. mole-’ at 290 rnM (1). Sodium salts of ADP, ATP, ITP, UTP, and crystalline disodium ATP were obtained from the Pabst Laboratories, Milwaukee, Wisconsin. Myokinase was prepared according to the procedure of Colowick and Kalckar (3), and adenylic acid deaminase according to the procedure of Kalckar (4). Alcohol dehydrogenase was obtained from the Worthington Biochemical Corporation, Freehold, New Jersey. Alumina C-r was prepared according to the procedure of Willstatter and Kraut (5).